Effects of urbanization and accessibility to sanitation services on water quality in urban streams in Uruguay.

The world's urban population is growing rapidly, and threatening natural ecosystems, especially streams. Urbanization leads to stream alterations, increased peak flow frequencies, and reduced water quality due to pollutants, morphological changes, and biodiversity loss, known as the urban stream syndrome. However, a shift towards recognizing urban streams as valuable natural systems is occurring, emphasizing green infrastructure and nature-based solutions. This study in Uruguay examined water quality in various watersheds with different urbanization levels and socio-environmental characteristics along a precipitation gradient. Using Geographic Information Systems (GIS) and in situ data, we assessed physicochemical parameters, generated territorial variables, and identified key predictors of water quality. We found that urbanization, particularly urban areas, paved areas, and populations without sanitation, significantly influenced water quality parameters. These factors explained over 50% of the variation in water quality indicators. However, the relationship between urbanization and water quality was non-linear, with abrupt declines after specific urban intensity thresholds. Our results illustrate that ensuring sanitation networks and managing green areas effectively are essential for preserving urban stream water quality. This research underscores the importance of interdisciplinary teams and localized data for informed freshwater resource management.

Evaluation of low-cost viral concentration methods in wastewaters: Implications for SARS-CoV-2 pandemic surveillances.

In the pandemic of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) many strategies have been performed in order to control viral spread in the population and known the real-time situation about the number of infected persons. In this sense, Wastewater Based Epidemiology (WBE) has been applied as an excellent tool to evaluate the virus circulation in a population. In order to obtain reliable results, three low-cost viral concentration methods were evaluated in this study, polyethylene glycol (PEG) precipitation, skimmed milk flocculation (SM) and Aluminum polychloride flocculation, for Pseudomonas aeruginosa bacteriophage PP7 as a surrogate for non-enveloped viruses and Bovine Coronavirus (BCoV) as a surrogate for enveloped virus, with emphasis for SARS- CoV-2. Our results suggest that PEG precipitation for viral concentration, for both enveloped and non-enveloped virus from wastewater is an appropriate approach since it was more sensitive compared to SM flocculation and Aluminum polychloride flocculation. This methodology can be used for WBE studies in order to follow the epidemiology of the SARS-CoV-2 pandemic, mainly in developing countries where the economic resources are frequently limited.

Correction to: Water quality evaluation of two urban streams in Northwest Uruguay: are national regulations for urban stream quality sufficient?

The original version of this article unfortunately contained an error in the published paper.

Water quality evaluation of two urban streams in Northwest Uruguay: are national regulations for urban stream quality sufficient?

Poor water quality in urban streams places at risk the health of urban residents and the integrity of urban environments, ultimately leading to the "urban stream syndrome." In response to growing concerns regarding urban streams in Uruguay, we evaluated the spatiotemporal variation in water quality parameters in two urban streams, the Ceibal and La Curtiembre streams, over 12-18 months. A proposal for an Urban Water Quality Index (UWQI) was developed based on national water quality standards for two stream classes to assess variability in overall stream condition over time. Both streams displayed extremely high levels of fecal coliform concentrations in mid-downstream sites and relatively high levels of total dissolved phosphorus and ammonia well above the national standards of 0.025 mg/L and 0.02 mg/L, respectively. Nitrate was consistently below the national maximum of 44.3 mg/L, calling to question the adequacy of this standard for designated uses. Over 40% of samples had oxygen levels below the national standard of 5 mg/L, and a dead zone (average 1.5 mg/L) was detected in the downstream reach of the La Curtiembre stream. Despite differences in land use and urban context, monthly observations of nutrients and coliforms indicated high levels of contamination in mid-downstream reaches, which could present a health risk for the populations in Paysandú and Salto. This study highlights the degradation of urban streams in two major cities in Uruguay and the need for a comparative diagnosis of stream condition as a basis for decision-making regarding urban development and water resources.

Toward the understanding of the molecular basis for the inhibition of COX-1 and COX-2 by phenolic compounds present in Uruguayan propolis and grape pomace.

Propolis and grape pomace have significant amounts of phenols which can take part in anti-inflammatory mechanisms. As the cyclooxygenases 1 and 2 (COX-1 and COX-2) are involved in said mechanisms, the possibility for a selective inhibition of COX-2 was analyzed in vitro and in silico. Propolis and grape pomace from Uruguayan species were collected, extracted in hydroalcoholic mixture and analyzed. Based on phenols previously identified, and taking as reference the crystallographic structures of COX-1 and COX-2 in complex with the commercial drug Celecoxib, a molecular docking procedure was devised to adjust 123 phenolic molecular models at the enzyme-binding sites. The most important results of this work are that the extracts have an overall inhibition activity very similar in COX-1 and COX-2, i.e. they do not possess selective inhibition activity for COX-2. Nevertheless, 10 compounds of the phenolic database turned out to be more selective and 94 phenols resulted with similar selectivity than Celecoxib, an outcome that accounts for the overall experimental inhibition measures. Binding site environment observations showed increased polarity in COX-2 as compared with COX-1, suggesting that polarity is the key for selectivity. Accordingly, the screening of molecular contacts pointed to the residues: Arg106, Gln178, Leu338, Ser339, Tyr341, Tyr371, Arg499, Ala502, Val509, and Ser516, which would explain, at the atomic level, the anti-inflammatory effect of the phenolic compounds. Among them, Gln178 and Arg499 appear to be essential for the selective inhibition of COX-2.